parent strain trichoderma reesei tu 6 Search Results


jecorina  (ATCC)
93
ATCC jecorina
Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with <t>the</t> <t>pyr4</t> gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.
Jecorina, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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kh2po4 tu 6-09-5324-87  (Chimmed Inc)
90
Chimmed Inc kh2po4 tu 6-09-5324-87
Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with <t>the</t> <t>pyr4</t> gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.
Kh2po4 Tu 6 09 5324 87, supplied by Chimmed Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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pyr4 negative mutant tu 6  (ATCC)
90
ATCC pyr4 negative mutant tu 6
Characterization of H. jecorina lad1 and lad1 xdh1 deletion mutants. (A) Southern analysis of lad1 deletion strains. Genomic DNAs of strain QM9414 and lad1 deletion strains were digested with BamHI and probed with a 1-kb BamHI-MluI fragment of lad1. In the lad1 deletion strains, replacement of the lad1 coding region by the H. jecorina <t>pyr4</t> marker leads to a 1.7-kb increase in the size of the hybridizing fragment. This change leads to an increase in the size of the 3-kb hybridizing fragment in strain QM9414 to an ∼4.7-kb fragment in the lad1 deletion strains. (B) Southern analysis of lad1 xdh1 deletion strains, performed as described in the legend to Fig. ​Fig.6A.6A. (C) Comparison of the growth behaviors of parent strain QM9414 and deletion strains on plates containing d-glucose (Glc), d-xylose (Xyl), and xylitol (Xol) as carbon sources. (D) Northern analysis of lad1 and xdh1 transcript levels in strain QM9414 and deletion strains. Samples were obtained 6 h after transfer from a glycerol culture (Gly) to l-arabinose (Ara) and d-xylose (Xyl) cultures.
Pyr4 Negative Mutant Tu 6, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti ace tubulin  (Santa Cruz Biotechnology)
96
Santa Cruz Biotechnology anti ace tubulin
Characterization of H. jecorina lad1 and lad1 xdh1 deletion mutants. (A) Southern analysis of lad1 deletion strains. Genomic DNAs of strain QM9414 and lad1 deletion strains were digested with BamHI and probed with a 1-kb BamHI-MluI fragment of lad1. In the lad1 deletion strains, replacement of the lad1 coding region by the H. jecorina <t>pyr4</t> marker leads to a 1.7-kb increase in the size of the hybridizing fragment. This change leads to an increase in the size of the 3-kb hybridizing fragment in strain QM9414 to an ∼4.7-kb fragment in the lad1 deletion strains. (B) Southern analysis of lad1 xdh1 deletion strains, performed as described in the legend to Fig. ​Fig.6A.6A. (C) Comparison of the growth behaviors of parent strain QM9414 and deletion strains on plates containing d-glucose (Glc), d-xylose (Xyl), and xylitol (Xol) as carbon sources. (D) Northern analysis of lad1 and xdh1 transcript levels in strain QM9414 and deletion strains. Samples were obtained 6 h after transfer from a glycerol culture (Gly) to l-arabinose (Ara) and d-xylose (Xyl) cultures.
Anti Ace Tubulin, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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type strain trichoderma reesei tu 6  (ATCC)
95
ATCC type strain trichoderma reesei tu 6
Characterization of H. jecorina lad1 and lad1 xdh1 deletion mutants. (A) Southern analysis of lad1 deletion strains. Genomic DNAs of strain QM9414 and lad1 deletion strains were digested with BamHI and probed with a 1-kb BamHI-MluI fragment of lad1. In the lad1 deletion strains, replacement of the lad1 coding region by the H. jecorina <t>pyr4</t> marker leads to a 1.7-kb increase in the size of the hybridizing fragment. This change leads to an increase in the size of the 3-kb hybridizing fragment in strain QM9414 to an ∼4.7-kb fragment in the lad1 deletion strains. (B) Southern analysis of lad1 xdh1 deletion strains, performed as described in the legend to Fig. ​Fig.6A.6A. (C) Comparison of the growth behaviors of parent strain QM9414 and deletion strains on plates containing d-glucose (Glc), d-xylose (Xyl), and xylitol (Xol) as carbon sources. (D) Northern analysis of lad1 and xdh1 transcript levels in strain QM9414 and deletion strains. Samples were obtained 6 h after transfer from a glycerol culture (Gly) to l-arabinose (Ara) and d-xylose (Xyl) cultures.
Type Strain Trichoderma Reesei Tu 6, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with the pyr4 gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.

Journal: BMC Biology

Article Title: Light-dependent roles of the G-protein α subunit GNA1 of Hypocrea jecorina (anamorph Trichoderma reesei )

doi: 10.1186/1741-7007-7-58

Figure Lengend Snippet: Gene structure of gna1 , deletion cassette and construct for transformation with the gna1 Q204L allele . (a) The gene structure of gna1 is shown along with its exons and introns and the predicted GNA1 protein. (b) Construction of a transformation cassette for deletion of gna1 by replacing its open reading frame with the pyr4 gene as a selection marker. (c) Strategy for mutational activation of GNA1 by construction of a transformation cassette comprising a single amino acid exchange at position 204 as indicated by a triangle. The restriction sites Sal I and Apa I have been introduced using nested primers to facilitate cloning.

Article Snippet: The uridine auxotrophic H. jecorina ( T. reesei ) strain TU-6 (ATCC MYA-256; Δ pyr4 ; [ ]) was used for construction of the recombinant strains described in this study and maintained on malt extract agar supplemented with 10 mM uridine (Sigma-Aldrich, Madison, WI, USA).

Techniques: Construct, Transformation Assay, Selection, Marker, Activation Assay, Cloning

Characterization of H. jecorina lad1 and lad1 xdh1 deletion mutants. (A) Southern analysis of lad1 deletion strains. Genomic DNAs of strain QM9414 and lad1 deletion strains were digested with BamHI and probed with a 1-kb BamHI-MluI fragment of lad1. In the lad1 deletion strains, replacement of the lad1 coding region by the H. jecorina pyr4 marker leads to a 1.7-kb increase in the size of the hybridizing fragment. This change leads to an increase in the size of the 3-kb hybridizing fragment in strain QM9414 to an ∼4.7-kb fragment in the lad1 deletion strains. (B) Southern analysis of lad1 xdh1 deletion strains, performed as described in the legend to Fig. ​Fig.6A.6A. (C) Comparison of the growth behaviors of parent strain QM9414 and deletion strains on plates containing d-glucose (Glc), d-xylose (Xyl), and xylitol (Xol) as carbon sources. (D) Northern analysis of lad1 and xdh1 transcript levels in strain QM9414 and deletion strains. Samples were obtained 6 h after transfer from a glycerol culture (Gly) to l-arabinose (Ara) and d-xylose (Xyl) cultures.

Journal:

Article Title: d -Xylose Metabolism in Hypocrea jecorina : Loss of the Xylitol Dehydrogenase Step Can Be Partially Compensated for by lad1 -Encoded l -Arabinitol-4-Dehydrogenase

doi: 10.1128/EC.2.5.867-875.2003

Figure Lengend Snippet: Characterization of H. jecorina lad1 and lad1 xdh1 deletion mutants. (A) Southern analysis of lad1 deletion strains. Genomic DNAs of strain QM9414 and lad1 deletion strains were digested with BamHI and probed with a 1-kb BamHI-MluI fragment of lad1. In the lad1 deletion strains, replacement of the lad1 coding region by the H. jecorina pyr4 marker leads to a 1.7-kb increase in the size of the hybridizing fragment. This change leads to an increase in the size of the 3-kb hybridizing fragment in strain QM9414 to an ∼4.7-kb fragment in the lad1 deletion strains. (B) Southern analysis of lad1 xdh1 deletion strains, performed as described in the legend to Fig. ​Fig.6A.6A. (C) Comparison of the growth behaviors of parent strain QM9414 and deletion strains on plates containing d-glucose (Glc), d-xylose (Xyl), and xylitol (Xol) as carbon sources. (D) Northern analysis of lad1 and xdh1 transcript levels in strain QM9414 and deletion strains. Samples were obtained 6 h after transfer from a glycerol culture (Gly) to l-arabinose (Ara) and d-xylose (Xyl) cultures.

Article Snippet: The H. jecorina parent strains used in this study were QM9414 (ATCC 26921) and the pyr4 -negative mutant TU-6 (ATCC MYA-256) ( 7 ).

Techniques: Marker, Northern Blot